Human UGT1A1 ELISA Kit

$696.00

  • Model: ELA-E12952h
  • 20 Units in Stock
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Human UGT1A1 ELISA Kit

Product Name:Human UGT1A1 ELISA Kit
Packing:96T

Catalog No.:ELA-E12952h

Gene Name:UGT1A1

Detect Range:0.156-10 ng/mL

Sensitivity:0.045ng/ml

Target Protein Name:Human UDP-glucuronosyltransferase 1-1

Alternative Name:UDP-glucuronosyltransferase 1-1, UDPGT 1-1, UGT1*1, UGT1-01, UGT1.1, Bilirubin-specific UDPGT isozyme 1, hUG-BR1, UDP-glucuronosyltransferase 1-A, UGT-1A, UGT1A, UDP-glucuronosyltransferase 1A1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human UGT1A1 ELISA Kit allows for the in vitro quantitative determination of Human UDP-glucuronosyltransferase 1-1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human UGT1A1 ELISA Kit has been pre-coated with an UGT1A1 antibody specific to Human UDP-glucuronosyltransferase 1-1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human UDP-glucuronosyltransferase 1-1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human UDP-glucuronosyltransferase 1-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human UDP-glucuronosyltransferase 1-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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