Human UMOD ELISA Kit

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  • Model: ELA-E2280h
  • 20 Units in Stock
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Human UMOD ELISA Kit

Product Name:Human UMOD ELISA Kit
Packing:96T

Catalog No.:ELA-E2280h

Gene Name:UMOD

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/mL

Target Protein Name:Human Uromodulin

Alternative Name:Uromodulin, Tamm-Horsfall urinary glycoprotein, THP, UMOD
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human UMOD ELISA Kit allows for the in vitro quantitative determination of Human Uromodulin concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human UMOD ELISA Kit has been pre-coated with an UMOD antibody specific to Human Uromodulin .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Uromodulin and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Uromodulin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Uromodulin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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