Human VIPR2 ELISA Kit

Product Name:Human VIPR2 ELISA Kit
Packing:96T

Catalog No.:ELA-E15102h

Gene Name:VIPR2

Detect Range:0.156-10 ng/mL

Sensitivity:0.078ng/ml

Target Protein Name:Human Vasoactive intestinal polypeptide receptor 2

Alternative Name:Vasoactive intestinal polypeptide receptor 2, VIP-R-2, Helodermin-preferring VIP receptor, Pituitary adenylate cyclase-activating polypeptide type III receptor, PACAP type III receptor, PACAP-R-3, PACAP-R3
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Human VIPR2 ELISA Kit allows for the in vitro quantitative determination of Human Vasoactive intestinal polypeptide receptor 2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human VIPR2 ELISA Kit has been pre-coated with an VIPR2 antibody specific to Human Vasoactive intestinal polypeptide receptor 2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Vasoactive intestinal polypeptide receptor 2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Vasoactive intestinal polypeptide receptor 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human Vasoactive intestinal polypeptide receptor 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!