Human Vitamin D ELISA Kit

Starting at: $696.00

  • Model: ELA-E0917h
  • 20 Units in Stock
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Human Vitamin D,VD ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY!

 

Alternative name

Human VD ELISA KIT,Human Vitamin D ELISA KIT

 

Intended use

Human VD ELISA KIT allows for the in vitro quantitative determination of Human Vitamin D,VD concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

 

Test principle

Human Vitamin D ELISA KIT is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to VD During the reaction, VD in the sample or standard competes with a fixed amount of biotin-labeled VD for sites on a pre-coated Monoclonal antibody specific to VD. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of VD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 1.56-100 ng/mL

PDF Manual download

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