• Model: EF002692
  • 100 Units in Stock
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Catalog  EF002692

Packing 48T/96T

Reactivity Human

Detect Range 15.625-1000pg/ml

NEF3 ELISA KIT Sensitivity< 9.375pg/ml

Application NEF3 ELISA KIT|Human  is for detecting the concentration of Human NEF3 in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade


Principle of NEF3 ELISA KIT|Human

The microtiter plate provided in NEF3 ELISA KIT|Human has been pre-coated with an Human NEF3 antibody specific to Human NEF3 . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human NEF3 antibody preparation specific for Human NEF3 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human NEF3 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human NEF3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.


*Please noted that NEF3 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!

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