NT-proBNP ELISA KIT|Human

Catalog  EF000264

Packing 48T/96T

Reactivity Human

Detect Range 39.063-2500pg/ml

NT-proBNP ELISA KIT Sensitivity< 23.438pg/ml

Application NT-proBNP ELISA KIT|Human  is for detecting the concentration of Human NT-proBNP in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade

Principle of NT-proBNP ELISA KIT|Human

The microtiter plate provided in NT-proBNP ELISA KIT|Human has been pre-coated with an Human NT-proBNP antibody specific to Human NT-proBNP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human NT-proBNPantibody preparation specific for Human NT-proBNP then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human NT-proBNP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human NT-proBNPin the samples is then determined by comparing the O.D. of the samples to the standard curve.

*Please noted that NT-proBNP ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!