TPO-Ab ELISA KIT|Human
Catalog EF007224
Packing 48T/96T
Reactivity Human
Detect Range 0.781-50IU/ml
TPO-Ab ELISA KIT Sensitivity< 0.469IU/ml
Application TPO-Ab ELISA KIT|Human is for detecting the concentration of Human TPO-Ab in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of TPO-Ab ELISA KIT|Human
The microtiter plate provided in TPO-Ab ELISA KIT|Human has been pre-coated with an Human TPO-Ab antibody specific to Human TPO-Ab . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human TPO-Ab antibody preparation specific for Human TPO-Ab then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human TPO-Ab , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human TPO-Ab in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that TPO-Ab ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!