Rat Nibrin, NBN ELISA Kit

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

NBN,NBS1,P95,nbs,nibrin; Nijmegen breakage syndrome 1; AT-V1; AT-V2; ATV; NBS; P95; cell cycle regulatory protein p95; p95 protein of the MRE11/RAD50 complex,Cell cycle regulatory protein p95;Nijmegen breakage syndrome protein 1, Nijmegen breakage syndrome protein 1 homolog

Search name

Rat NBN ELISA KIT ,Rat NBS1 ELISA KIT ,Rat P95 ELISA KIT ,Rat nbs ELISA KIT ,Rat nibrin ELISA KIT ,Rat Nijmegen breakage syndrome 1 ELISA KIT ,Rat AT-V1 ELISA KIT ,Rat AT-V2 ELISA KIT ,Rat ATV ELISA KIT ,Rat NBS ELISA KIT ,Rat P95 ELISA KIT ,Rat cell cycle regulatory protein p95 ELISA KIT ,Rat p95 protein of the MRE11/RAD50 complex ELISA KIT ,Rat Cell cycle regulatory protein p95 ELISA KIT ,Rat Nijmegen breakage syndrome protein 1 ELISA KIT ,Rat Nijmegen breakage syndrome protein 1 homolog ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Nibrin, NBN concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nibrin, During the reaction, Nibrin in the sample or standard competes with a fixed amount of biotin-labeled Nibrin for sites on a pre-coated Monoclonal antibody specific to Nibrin. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Nibrin in the samples is then determined by comparing the O.D. of the samples to the standard curve.