Rat AGAP2 ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  AGAP2

Protein Name  Arf-GAP with GTPase, ANK repeat and PH domain-containing protein 2

Alternative Name

Rat AGAP2 ELISA KIT ,Rat CENTG1 ELISA KIT ,Rat GGAP2 ELISA KIT ,Rat PIKE ELISA KIT ,Rat arf-GAP with GTPase ELISA KIT ,Rat  ANK repeat and PH domain-containing protein 2 ELISA KIT ,Rat Arf GAP with GTP-binding protein-like ELISA KIT ,Rat  ANK repeat and PH domains 2 ELISA KIT ,Rat GTP-binding and GTPase activating protein 2 ELISA KIT ,Rat centaurin ELISA KIT ,Rat  gamma 1 ELISA KIT ,Rat cnt-g1 ELISA KIT ,Rat phosphatidylinositol 3-kinase enhancer ELISA KIT ,Rat phosphoinositide 3-kinase enhancer ELISA KIT ,Rat ArfGAP with GTPase domain ankyrin repeat and PH domain 2 ELISA KIT ,

Intended use

Rat AGAP2 ELISA KIT allows for the in vitro quantitative determination of AGAP2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Test principle      

The microtiter plate provided in Rat AGAP2 ELISA KIT has been pre-coated with an AGAP2 antibody specific to AGAP2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for AGAP2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain AGAP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AGAP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20