Anti-CASP3 antibody
Product Information
Product Name: Anti-CASP3 antibody
Catalog No.: LSMab09938
Packing: 100ug
Form: liquid
Purity:more than 95% as determined by SDS-PAGE
Purification: protein A+G purified
Host: Mouse
Isotype: IgG1
Storage Condition: 0.01MPBS pH 7.4,-20degrees for 24 months(Avoid repeated freeze / thaw cycles.)
Immunogen Information
Immunogen Name:caspase 3, apoptosis-related cysteine peptidase
Background: Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase(PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol regulatory element binding proteins(SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6,-7 and-9. Caspase 3 is involved in the cleavage of huntingtin. Caspase 3 can also form heterocomplex with other proteins and performs MW of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.
Synonyms: Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1
Calculated MW: 32-35 kDa, 19 kDa, 17 kDa
Uniprot ID: P42574
Gene ID: 836
Validation
Specificity: Human, Mouse, Rat
Tested Application: ELISA, IHC, WB,IF
Recommended dilution: WB: 1:1000-1:5000; IHC: 1:100-1:500; IF: 1:50-1:500
IHC use: Immunohistochemistry of paraffin-embedded human breast cancer using LSMab09938(CASP3 antibody) at dilution of 1:200
WB use: Untreated and Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with LSMab09938 (CASP3 antibody) at dilution of 1:3000
Caution:
1. Product is used for research only!