Anti-Cytokeratin 19 antibody

Product Name Anti-Cytokeratin 19 antibody

Catalog No PAab02209

Packing 100ug

Form liquid

Alternative Name CK 19 antibody, CK19 antibody, Cytokeratin 19 antibody, K19 antibody, K1CS antibody, keratin 19 antibody, KRT19

Purification Protein A+G purification

Purity 95% as determined by SDS-PAGE

Host Mouse

Isotype IgG2a

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells. KRT19, one of type I keratins, is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Due to its high sensitivity, KRT19 is the most used marker for detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients. This antibody, generated against full length KRT19 protein, also recognizes KRT17, another type I keratin homologous to KRT19. KRT17 is used as a marker for trauma.

Immunogen keratin 19

Specificity Human,Mouse ,Rat

Tested Application ELISA,WB,IHC

Recommended dilution WB:1:500-1:5000;IHC:1:50-1:500

IHC use

Immunohistochemistry of paraffin-embedded human stomach cancer tissue slide using KRT19 Antibody at dilution of 1:200. heat mediated antigen retrieved with Tris-EDTA bufferpH9).

WB use

MCF-7 cells were subjected to SDS PAGE followed by western blot with PAab02209( KRT19 Antibody) at dilution of 1:1500

Protein Information

Gene ID3880

Uniprot ID P08727

Calculated MW 44kDa

location