PTPRN antibody
Product Name: PTPRN antibody
Catalog No PAab04093
Packing 100ug
Form liquid
Alternative Name
Purification Protein A+G purification
Purity 95% as determined by SDS-PAGE
Host Mouse
Isotype IgG1
Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)
Background/ FUNCTION
IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell). IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.
Immunogen protein tyrosine phosphatase, receptor type, N
Specificity Human
Tested Application ELISA,WB,IF,IHC
Recommended dilution
IHC use
Immunohistochemistry of paraffin-embedded human pancreas cancer tissue slide using PTPRN Antibody at dilution of 1:400 heat mediated antigen retrieved with Tris-EDTA bufferpH9).
WB use
human brain tissue were subjected to SDS PAGE followed by western blot with PAab04093(PTPRN antibody) at dilution of 1:500
Protein Information
Gene ID5798
Uniprot ID Q16849
Calculated MW 105 kDa
location
Plasma membrane
Cell membrane 1 Publication; Single-pass type I membrane protein By similarity
Endosome
Endosome By similarity
Other locations
Membrane By similarity; Single-pass type I membrane protein By similarity
secretory vesicle membrane 1 Publication; Single-pass type I membrane protein Curated
Perikaryon By similarity
axon By similarity
synapse By similarity
Note: Detected on neuronal secretory vesicles, but not on synaptic vesicles. Colocalizes with insulin-containing secretory granules (PubMed:25561468). Primarily detected on secretory vesicle membranes. Transiently found at the cell membrane, when secretory vesicles fuse with the cell membrane to release their cargo. Is then endocytosed and recycled to secretory vesicles via the Golgi apparatus membranes.By similarity1 Publication
ICA512-transmembrane fragment :
Other locations
secretory vesicle membrane By similarity
ICA512-cleaved cytosolic fragment :
Nucleus
Nucleus 1 Publication