Anti-MSH6 antibody

$300.00

  • Model: PAab05376
  • 50 Units in Stock
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Anti-MSH6 antibody

Product Name Anti-MSH6 antibody

Catalog No PAab05376

Packing 100ug

Form liquid

Alternative Name GTBP

Purification Protein A+G purification

Purity 95% as determined by SDS-PAGE

Host Mouse

Isotype IgG2a

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

Immunogen mutS homolog 6 (E. coli)

Specificity Human

Tested Application ELISA,WB,IHC,IP

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using MSH6 Antibody at dilution of 1:800 heat mediated antigen retrieved with Tris-EDTA bufferpH9).

WB use

HEK-293 cells were subjected to SDS PAGE followed by western blot with PAab05376( MSH6 Antibody) at dilution of 1:1000

Protein Information

Gene ID2956

Uniprot ID P52701

Calculated MW

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