Anti-PCNA antibody

Product Name Anti-PCNA antibody

Catalog No PAab06217

Packing 100ug

Form liquid

Alternative Name

Purification Protein A+G purification

Purity 95% as determined by SDS-PAGE

Host Mouse

Isotype IgG1

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Immunogen proliferating cell nuclear antigen

Specificity Human, Mouse

Tested Application ELISA,WB,IHC,FC,IP

Recommended dilution

IHC use

Immunohistochemistry of paraffin-embedded human breast cancer usingPCNA antibody at dilution of 1:800

WB use

HEK-293 cells were subjected to SDS PAGE followed by western blot with PAab06217( PCNA Antibody) at various dilution.

Protein Information

Gene ID5111

Uniprot ID P12004

Calculated MW

location