Anti-WTAP antibody

Product Name Anti-WTAP antibody

Catalog No PAab09530

Packing 100ug

Form liquid

Alternative Name DKFZp686F20131 antibody, Female lethal(2)D homolog antibody, hFL(2)D antibody, KIAA0105 antibody, WT1 associated protein antibody, WTAP

Purification Protein A+G purification

Purity 95% as determined by SDS-PAGE

Host Mouse

Isotype IgG1

Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20Centigrade for 24 months (Avoid repeated freeze / thaw cycles.)

Background/ FUNCTION

Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).

Immunogen Wilms tumor 1 associated protein

Specificity Human, Mouse

Tested Application ELISA,WB,IHC

Recommended dilution WB:1:1000-1:10000;IF:1:20-1:200;IHC:1:20-1:200

IHC use

Immunohistochemistry of paraffin-embedded human cervical cancer usingWTAP antibody at dilution of 1:50

WB use

HEK-293 cells were subjected to SDS PAGE followed by western blot with PAab09530( WTAP Antibody) at dilution of 1:2000

Protein Information

Gene ID9589

Uniprot ID Q15007

Calculated MW 49kDa

location