Mouse 12-hydroxyeicosatetraenoic acid ELISA KIT
96 Tests
Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
12-Hydroxyeicosatetraenoic acid,12-HETE

Search name
Mouse 12-Hydroxyeicosatetraenoic acid ELISA Kit, Mouse 12-HETE ELISA Kit

Intended use
Mouse 12-Hydroxyeicosatetraenoic acid ELISA Kit allows for the in vitro quantitative determination of 12-hydroxyeicosatetraenoic acid,12-Hydroxyeicosatetraenoic
acid concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
Mouse 12-Hydroxyeicosatetraenoic acid ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to 12-Hydroxyeicosatetraenoic acid, During the reaction, 12-Hydroxyeicosatetraenoic acid in the
sample or standard competes with a fixed amount of biotin-labeled 12-Hydroxyeicosatetraenoic acid for sites on a pre-coated Monoclonal
antibody specific to 12-Hydroxyeicosatetraenoic acid. Excess conjugate and unbound sample or standard are washed from the plate.
Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution
is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of 12-Hydroxyeicosatetraenoic acid in the
samples is then determined by comparing the O.D. of the samples to the standard curve.

Mouse 12-Hydroxyeicosatetraenoic acid ELISA Kit Range 0.15-10ng/ml

Mouse 12-Hydroxyeicosatetraenoic acid ELISA Kit PDF manual download

Order 12-Hydroxyeicosatetraenoic acid ELISA Kit by [email protected]