Mouse Acyl- CoA- binding protein, Dbi ELISA KIT
Product Name:Mouse Acyl- CoA- binding protein, Dbi ELISA KIT
Packing:96T
Catalog No.:ELI-35108m
Gene Name:Mouse Dbi
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Dbi
Alternative Name:Mouse Acyl-CoA-binding protein, Dbi
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Acyl- CoA- binding protein, Dbi ELISA KIT allows for the in vitro quantitative determination of Mouse Dbi concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Acyl- CoA- binding protein, Dbi ELISA KIT has been pre-coated with an Mouse Acyl-CoA-binding protein, Dbi antibody specific to Mouse Dbi .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Dbi and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Dbi , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Dbi in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!