Mouse Alpha- 1- antitrypsin 1- 5, Serpina1e ELISA KIT
Product Name:Mouse Alpha- 1- antitrypsin 1- 5, Serpina1e ELISA KIT
Packing:96T
Catalog No.:ELI-49638m
Gene Name:Mouse Serpina1e
Detect Range:78.1-5000pg/ml
Sensitivity:46.875pg/ml
Target Protein Name:Mouse Serpina1e
Alternative Name:Mouse Alpha-1-antitrypsin 1-5, Serpina1e
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Alpha- 1- antitrypsin 1- 5, Serpina1e ELISA KIT allows for the in vitro quantitative determination of Mouse Serpina1e concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Alpha- 1- antitrypsin 1- 5, Serpina1e ELISA KIT has been pre-coated with an Mouse Alpha-1-antitrypsin 1-5, Serpina1e antibody specific to Mouse Serpina1e .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Serpina1e and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Serpina1e , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Serpina1e in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!