Mouse Cyclic Diguanylate, CdiGMP ELISA Kit

Starting at: $736.00

  • Model: CELI-66105m
  • 20 Units in Stock
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Mouse Cyclic Diguanylate, CdiGMP ELISA Kit

96 Tests

FOR RESEARCH USE ONLY!

Synonyms
Cyclic Diguanylate,CdiGMP; c-di-GMP

Search name

Mouse CdiGMP ELISA Kit,Mouse Cyclic Diguanylate ELISA Kit, Cyclic Diguanylate ELISA Kit,CdiGMP ELISA Kit

Intended use
Mouse CdiGMP ELISA Kit allows for the in vitro quantitative determination of mouse Cyclic Diguanylate,CdiGMP concentrations in serum,plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
Mouse CdiGMP ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CdiGMP, During the reaction, CdiGMP in the sample or standard competes with a fixed amount of biotin-labeled CdiGMP for sites on a pre-coated Monoclonal antibody specific to CdiGMP. Excess conjugate and unbound sample or
standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CdiGMP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Mouse CdiGMP ELISA Kit Range 0.78-50 ng/ml

Mouse CdiGMP ELISA Kit PDF manual download

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