Mouse QDPR ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  QDPR

Protein Name  quinoid dihydropteridine reductase

Alternative Name

Mouse Qdpr ELISA KIT ,Mouse dihydropteridine reductase ELISA KIT ,Mouse DHPR ELISA KIT ,Mouse PKU2 ELISA KIT ,Mouse SDR33C1 ELISA KIT ,Mouse 6 ELISA KIT ,Mouse 7-dihydropteridine reductase ELISA KIT ,Mouse HDHPR ELISA KIT ,Mouse short chain dehydrogenase/reductase family 33C member 1 ELISA KIT ,Mouse testis secretory sperm-binding protein Li 236P ELISA KIT ,Mouse quinoid dihydropteridine reductase ELISA KIT

Intended use

Mouse QDPR ELISA KIT allows for the in vitro quantitative determination of QDPR concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Test principle      

The microtiter plate provided in Mouse QDPR ELISA KIT has been pre-coated with an QDPR antibody specific to QDPR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for QDPR and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain QDPR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of QDPR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20