Mouse E3 ubiquitin- protein ligase ZNRF1, Znrf1 ELISA KIT

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  • Model: ELI-17685m
  • 20 Units in Stock
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Mouse E3 ubiquitin- protein ligase ZNRF1, ZNRF1 ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

ZNRF1,E3 ubiquitin-protein ligase ZNRF1; NIN283; nerve injury gene 283; nerve injury-induced gene 283 protein; zinc and ring finger protein 1; zinc/RING finger protein 1; zinc and ring finger 1, E3 ubiquitin protein ligase

 

Search name

Mouse ZNRF1 ELISA KIT ,Mouse E3 ubiquitin-protein ligase ZNRF1 ELISA KIT ,Mouse NIN283 ELISA KIT ,Mouse nerve injury gene 283 ELISA KIT ,Mouse nerve injury-induced gene 283 protein ELISA KIT ,Mouse zinc and ring finger protein 1 ELISA KIT ,Mouse zinc/RING finger protein 1 ELISA KIT ,Mouse zinc and ring finger 1 ELISA KIT ,Mouse E3 ubiquitin protein ligase ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse E3 ubiquitin- protein ligase ZNRF1, ZNRF1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to E3 ubiquitin- protein ligase ZNRF1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for E3 ubiquitin- protein ligase ZNRF1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain E3 ubiquitin- protein ligase ZNRF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of E3 ubiquitin- protein ligase ZNRF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

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Delivery time 4-6month

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