Mouse Hemoglobin subunit alpha, HBA ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Hemoglobin subunit alpha,Alpha-globin,Hemoglobin alpha chain,HBA1,hemoglobin subunit alpha; HBH; alpha one globin; alpha-1 globin; alpha-1-globin; alpha-2 globin chain; alpha-globin; delta globin; hemoglobin alpha 1 globin chain; hemoglobin alpha chain; hemoglobin alpha-1 chain; hemoglobin, alpha 1
Search name
Mouse Hemoglobin subunit alpha ELISA KIT ,Mouse Alpha-globin ELISA KIT ,Mouse Hemoglobin alpha chain ELISA KIT ,Mouse HBA1 ELISA KIT ,Mouse hemoglobin subunit alpha ELISA KIT ,Mouse HBH ELISA KIT ,Mouse alpha one globin ELISA KIT ,Mouse alpha-1 globin ELISA KIT ,Mouse alpha-1-globin ELISA KIT ,Mouse alpha-2 globin chain ELISA KIT ,Mouse alpha-globin ELISA KIT ,Mouse delta globin ELISA KIT ,Mouse hemoglobin alpha 1 globin chain ELISA KIT ,Mouse hemoglobin alpha chain ELISA KIT ,Mouse hemoglobin alpha-1 chain ELISA KIT ,Mouse hemoglobin alpha 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Mouse Hemoglobin subunit alpha concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hemoglobin subunit alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hemoglobin subunit alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hemoglobin subunit alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Hemoglobin subunit alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
