Mouse HHIP- like protein 1, Hhipl1 ELISA KIT

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  • Model: ELI-48652m
  • 20 Units in Stock
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Mouse HHIPL1 ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  HHIPL1

Protein Name  HHIP- like protein 1

Alternative Name

Mouse HHIPL1 ELISA KIT ,Mouse KIAA1822 ELISA KIT ,Mouse UNQ9245 ELISA KIT ,Mouse HHIP-like protein 1 ELISA KIT ,Mouse ARAR9245 ELISA KIT ,Mouse HHIP like 1 ELISA KIT

Mouse HHIPL1 ELISA Kit Range 0.31-20ng/ml

Intended use

Mouse HHIPL1 ELISA KIT allows for the in vitro quantitative determination of HHIPL1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

 

Reagent

Quantity

Assay plate

1

Standard

2

Sample Diluent

1 × 20mL

Assay Diluent A

1 × 10mL

Assay Diluent B

1 × 10mL

Detection Reagent A

1 × 120μL

Detection Reagent B

1 × 120μL

Wash Buffer(25 x concentrate)

1 × 30mL

Substrate

1 × 10mL

Stop Solution

1 × 10mL

Plate sealer

5

 

Test principle      

The microtiter plate provided in Mouse HHIPL1 ELISA KIT has been pre-coated with an HHIPL1 antibody specific to HHIPL1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HHIPL1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HHIPL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HHIPL1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20

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