Mouse Igh-VJ558 145 ELISA Kit

FOR RESEARCH USE ONLY!

96 Tests

Alternative name

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Intended use

Mouse Igh-VJ558 145 ELISA kit allows for the in vitro quantitative determination of Mouse Igh-VJ558 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

Mouse Igh-VJ558 145 ELISA kit Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Igh-VJ558 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Igh-VJ558, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Igh-VJ558 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Mouse Igh-VJ558 145 ELISA kit  Range 0.15-10ng/ml

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