Mouse Immunoglobulin G, IgG ELISA Kit

Starting at: $468.00

  • Model: ELA-E0544m
  • 20 Units in Stock
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Mouse Immunoglobulin G, IgG ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

Immunoglobulin Y, Immunoglobulin G, IgG,IgY, Immunoglobulin gamma

 

Search name

Mouse IgG ELISA Kit, Mouse Immunoglobulin G ELISA Kit, Mouse IgY ELISA Kit, Mouse Immunoglobulin Y ELISA Kit, Mouse Immunoglobulin gamma ELISA Kit

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse Immunoglobulin G concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Immunoglobulin G. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated monoclonal antibody preparation specific for Immunoglobulin G and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Immunoglobulin G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Immunoglobulin G in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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