Mouse Interleukin- 4 receptor subunit alpha, Il4r ELISA KIT
Product Name:Mouse Interleukin- 4 receptor subunit alpha, Il4r ELISA KIT
Packing:96T
Catalog No.:ELI-06531m
Gene Name:Mouse Il4r
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Il4r
Alternative Name:Mouse Interleukin-4 receptor subunit alpha, Il4r
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Interleukin- 4 receptor subunit alpha, Il4r ELISA KIT allows for the in vitro quantitative determination of Mouse Il4r concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Interleukin- 4 receptor subunit alpha, Il4r ELISA KIT has been pre-coated with an Mouse Interleukin-4 receptor subunit alpha, Il4r antibody specific to Mouse Il4r .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Il4r and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Il4r , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Il4r in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!