Mouse Macrosialin, CD68 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CD68,Macrosialin
Alternative name
Mouse CD68 ELISA KIT,Mouse Macrosialin ELISA KIT
Intended use
Mouse CD68 ELISA KIT allows for the in vitro quantitative determination of mouse macrosialin,Macrosialin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Mouse CD68 ELISA KIT has been pre-coated with an antibody specific to Macrosialin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Macrosialin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Macrosialin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Macrosialin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 0.78-50ng/ml
Mouse CD68 ELISA KIT PDF Manual download
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