Mouse Mitochondrial glutamate carrier 1, Slc25a22 ELISA KIT

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  • Model: ELI-32635m
  • 20 Units in Stock
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Mouse SLC25A22 ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  SLC25A22

Protein Name  Mitochondrial glutamate carrier 1

Alternative Name

Mouse SLC25A22 ELISA KIT ,Mouse EIEE3 ELISA KIT ,Mouse GC-1 ELISA KIT ,Mouse GC1 ELISA KIT ,Mouse NET44 ELISA KIT ,Mouse mitochondrial glutamate carrier 1 ELISA KIT ,Mouse glutamate/H(+) symporter 1 ELISA KIT ,Mouse solute carrier family 25 (mitochondrial carrier: glutamate) ELISA KIT ,Mouse  member 22 ELISA KIT ,Mouse solute carrier family 25 member 22 ELISA KIT

Intended use

Mouse SLC25A22 ELISA KIT allows for the in vitro quantitative determination of SLC25A22 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

 

Reagent

Quantity

Assay plate

1

Standard

2

Sample Diluent

1 × 20mL

Assay Diluent A

1 × 10mL

Assay Diluent B

1 × 10mL

Detection Reagent A

1 × 120μL

Detection Reagent B

1 × 120μL

Wash Buffer(25 x concentrate)

1 × 30mL

Substrate

1 × 10mL

Stop Solution

1 × 10mL

Plate sealer

5

 

Test principle      

The microtiter plate provided in Mouse SLC25A22 ELISA KIT has been pre-coated with an SLC25A22 antibody specific to SLC25A22. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SLC25A22 and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SLC25A22, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SLC25A22 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20

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