Mouse Mitofusin- 2, Mfn2 ELISA KIT

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  • Model: ELI-31284m
  • 20 Units in Stock
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Mouse MFN2 ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  MFN2

Protein Name  Mitofusin-2

Alternative Name

Mouse MFN2 ELISA KIT , Mouse transmembrane GTPase MFN2 ELISA KIT ,Mouse CMT2A ELISA KIT ,Mouse CMT2A2 ELISA KIT ,Mouse CMT2A2A ELISA KIT ,Mouse CMT2A2B ELISA KIT ,Mouse CPRP1 ELISA KIT ,Mouse HMSN6A ELISA KIT ,Mouse HSG ELISA KIT ,Mouse MARF ELISA KIT ,Mouse mitofusin-2 ELISA KIT ,Mouse hyperplasia suppressor ELISA KIT ,Mouse mitochondrial assembly regulatory factor ELISA KIT ,Mouse mitofusin 2 ELISA KIT

 

Intended use

Mouse MFN2 ELISA KIT allows for the in vitro quantitative determination of MFN2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

 

Reagent

Quantity

Assay plate

1

Standard

2

Sample Diluent

1 × 20mL

Assay Diluent A

1 × 10mL

Assay Diluent B

1 × 10mL

Detection Reagent A

1 × 120μL

Detection Reagent B

1 × 120μL

Wash Buffer(25 x concentrate)

1 × 30mL

Substrate

1 × 10mL

Stop Solution

1 × 10mL

Plate sealer

5

 

Test principle      

The microtiter plate provided in Mouse MFN2 ELISA KIT has been pre-coated with an MFN2 antibody specific to MFN2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for MFN2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MFN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MFN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20

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