Mouse monocyte chemotactic protein 1, MCP1 ELISA KIT

Mouse monocyte chemotactic protein 1, MCP1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

MCP-1, CCL2,small inducible cytokine A2; monocyte chemotactic protein 1; GDCF-2; HC11; HSMCR30; MCAF; MCP1; SCYA2; SMC-CF; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys-Cys), member 2; small-inducible cytokine A2; chemokine (C-C motif) ligand 2, C-C motif chemokine 2, Monocyte chemotactic protein 1

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse c-c motif chemokine 2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-C motif chemokine 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for C-C motif chemokine 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C-C motif chemokine 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of C-C motif chemokine 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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