Mouse monocyte chemotactic protein 1, MCP1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MCP-1, CCL2,small inducible cytokine A2; monocyte chemotactic protein 1; GDCF-2; HC11; HSMCR30; MCAF; MCP1; SCYA2; SMC-CF; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte secretory protein JE; small inducible cytokine subfamily A (Cys-Cys), member 2; small-inducible cytokine A2; chemokine (C-C motif) ligand 2, C-C motif chemokine 2, Monocyte chemotactic protein 1
Search name
Mouse MCP-1 ELISA KIT ,Mouse CCL2 ELISA KIT ,Mouse small inducible cytokine A2 ELISA KIT ,Mouse monocyte chemotactic protein 1 ELISA KIT ,Mouse GDCF-2 ELISA KIT ,Mouse HC11 ELISA KIT ,Mouse HSMCR30 ELISA KIT ,Mouse MCAF ELISA KIT ,Mouse MCP1 ELISA KIT ,Mouse SCYA2 ELISA KIT ,Mouse SMC-CF ELISA KIT ,Mouse monocyte chemoattractant protein 1 ELISA KIT ,Mouse monocyte chemoattractant protein-1 ELISA KIT ,Mouse monocyte chemotactic and activating factor ELISA KIT ,Mouse monocyte secretory protein JE ELISA KIT ,Mouse small inducible cytokine subfamily A (Cys-Cys) ELISA KIT ,Mouse member 2 ELISA KIT ,Mouse small-inducible cytokine A2 ELISA KIT ,Mouse chemokine (C-C motif) ligand 2 ELISA KIT ,Mouse C-C motif chemokine 2 ELISA KIT ,Mouse Monocyte chemotactic protein 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse c-c motif chemokine 2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to C-C motif chemokine 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for C-C motif chemokine 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain C-C motif chemokine 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of C-C motif chemokine 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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