Mouse Netrin- G1, Ntng1 ELISA KIT
Product Name:Mouse Netrin- G1, Ntng1 ELISA KIT
Packing:96T
Catalog No.:ELI-21352m
Gene Name:Ntng1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Mouse Netrin- G1
Alternative Name:Ntng1,Mouse Netrin- G1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Netrin- G1, Ntng1 ELISA KIT allows for the in vitro quantitative determination ofMouse Netrin- G1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Netrin- G1, Ntng1 ELISA KIT has been pre-coated with an Ntng1 antibody specific to Mouse Netrin- G1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Netrin- G1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Netrin- G1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Netrin- G1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!