Mouse Nuclear factor-kappa B, NF-κB ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Nuclear factor-kappa B,NF-κB,Nuclear factor NF-kappa-B p105 subunit,Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1,DNA-binding factor KBF1,EBP-1,

Search name

Mouse NF-κB ELISA KIT ,Mouse Nfkb ELISA KIT ,Mouse Nuclear factor-kappa B ELISA KIT ,Mouse Nuclear factor NF-kappa-B p105 subunit ELISA KIT ,Mouse Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 ELISA KIT ,Mouse DNA-binding factor KBF1 ELISA KIT ,Mouse EBP-1 ELISA KIT

Intended use

Mouse NF-κB ELISA KIT  allows for the in vitro quantitative determination of mouse Nuclear factor-kappa B,NF-κB concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Mouse NF-κB ELISA KIT  has been pre-coated with an antibody specific to NF-κB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NF-κB and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NF-κB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NF-κB in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Mouse NF-κB ELISA KIT  range 0.15-10ng/ml  

Mouse NF-κB ELISA KIT  manual download

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