Mouse Osteocalcin/ Bone gla protein, BGP ELISA Kit

Mouse Osteocalcin/Bone gla protein, BGP ELISA Kit
96 Tests 
Operating instruction
FOR RESEARCH USE ONLY; NOTFOR THERAPEUTIC OR DIAGNOSTICAPPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE 
BEGINNING! 
Synonyms 
Osteocalcin,Bone Gla protein(BGP),Gamma-carboxyglutamic acid-containing protein,; Osteocalcin,bone gamma-carboxyglutamate (gla) 
protein (osteocalcin); 
Search name 
Mouse Osteocalcin ELISA Kit, Mouse Bone gla protein ELISA Kit,Mouse BGP ELISA Kit, Mouse Gamma-carboxyglutamic 
acid-containing protein ELISA Kit, Mouse Osteocalcin bone gamma-carboxyglutamate (gla) protein ELISA Kit,
Intended use 
This immunoassay kit allows for the in vitro quantitative determination of mouse osteocalcin,Osteocalcinconcentrations inserum, Plasma, 
tissue homogenates and Cellculture supernates and Other biologicalfluids. 
T est principle 
The microtiter plate provided inthis kit has been pre-coated with an antibody specific to Osteocalcin. Standards or samples are then 
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparationspecific for Osteocalcin and Avidin 
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMBsubstrate solution is added to 
each well. Only those wells that contain Osteocalcin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in 
color. The enzyme-substrate reaction is terminated by the addition of asulphuric acid solution and the colorchange is measured 
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Osteocalcin in the samples is then determined by 
comparing the O.D. of the samples to the standard curve.

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