Mouse PtdIns (4, 5) P2, PIP2 ELISA Kit

CELI-66111m    96 Tests
Operating instructions

FOR RESEARCH USE ONLY!

Synonyms
Phosphatidylinositol 4,5-bisphosphate, PtdIns(4,5)P2, PIP2

Search name
Mouse PIP2 ELISA Kit,Mouse Phosphatidylinositol 4,5-bisphosphate ELISA Kit, Mouse PtdIns(4,5)P2 ELISA Kit,

Intended use
Mouse PIP2 ELISA Kit allows for the in vitro quantitative determination of Mouse Phosphatidylinositol 4,5-bisphosphate concentrations in
serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle
Mouse PIP2 ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Phosphatidylinositol 4,5-bisphosphate, During the reaction, Phosphatidylinositol 4,5-bisphosphate
in the sample or standard competes with a fixed amount of biotin-labeled Phosphatidylinositol 4,5-bisphosphate for sites on a pre-coated
Monoclonal antibody specific to Phosphatidylinositol 4,5-bisphosphate. Excess conjugate and unbound sample or standard are washed
from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB
substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the
color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Phosphatidylinositol
4,5-bisphosphate in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.78-50ng/ml

Mouse PIP2 ELISA Kit PDF manual download

Order Mouse PIP2 ELISA Kit by email:[email protected]

Caution:
Product is for research use only!
 

Mouse PIP2 ELISA Kit Reference

1. Zagazig J. Pharm. Sci. June, 2019 ISSN 1110-5089
Vol. 28, Issue 1, pp. 1- 12 ISSN (on-line) 2356_9786
Oleic acid antagonizes the action of high fructose high fat diet on insulin
secretion and adipose tissue β-arrestin signaling
Rehab M. E.-S Mansour1, *Islam A. A. E.-H. Ibrahim1, Hassan M. El-Fayoumi1,2, Ahmed Fahmy1
.
1Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Egypt.
2Department of Pharmacology and Toxicology, Faculty of Pharmacy, Kantara Sinai University, Egypt

2. Journal of Cardiovascular Pharmacology and Therapeutics
Article: Carvedilol Diminishes Cardiac Remodeling Induced by High-Fructose/High-Fat Diet in Mice via Enhancing Cardiac β-Arrestin2 Signaling
Authors:  Wael S. Ibrahim, BSc, Islam A. A. E.-H. Ibrahim, PhD, Mona F. Mahmoud, PhD, ...
First Published February 13, 2020 Research Article
https://doi.org/10.1177/1074248420905683

3.Effect of omega-3 fatty acids on glucose homeostasis: role of free fatty acid receptor 1

Shaimaa H. El-Fayoumi1 & Amr A. A. Mahmoud1,2 & Ahmed Fahmy1 & Islam A. A. E.-H. Ibrahim1
Received: 31 January 2020 /Accepted: 22 April 2020
# Springer-Verlag GmbH Germany, part of Springer Nature 2020

Naunyn-Schmiedeberg's Archives of Pharmacology
https://doi.org/10.1007/s00210-020-01883-5

4.Article: Quercetin and lithium chloride potentiate the protective effects of carvedilol against renal ischemia-reperfusion injury in high-fructose, high-fat diet-fed swiss albino mice independent of renal lipid signaling

Author：Asmaa M.RezkabIslam A.A.E.-H.IbrahimaMona F.MahmoudaAmr A.A.Mahmouda1

Received 6 September 2020, Revised 12 October 2020, Accepted 28 October 2020, Available online 4 November 2020.

Chemico-Biological Interactions
Available online 4 November 2020, 109307
In Press, Journal Pre-proofWhat are Journal Pre-proof articles?

doi: https://doi.org/10.1016/j.cbi.2020.109307