Mouse Pou5f1 ELISA KIT

Product Name:Mouse Pou5f1 ELISA KIT
Packing:96T

Catalog No.:ELI-05055m

Gene Name:Mouse Pou5f1

Detect Range:78.125-5000pg/ml

Sensitivity:46.875pg/ml

Target Protein Name:Mouse Pou5f1

Alternative Name:Mouse Pou5f1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Pou5f1 ELISA KIT allows for the in vitro quantitative determination of Mouse Pou5f1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Pou5f1 ELISA KIT has been pre-coated with an Mouse Pou5f1 antibody specific to Mouse Pou5f1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Pou5f1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Pou5f1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Pou5f1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!