Mouse FAM57A ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  FAM57A

Protein Name  Protein FAM57A

Alternative Name

Mouse FAM57A ELISA KIT ,Mouse protein FAM57A ELISA KIT ,Mouse CT120 ELISA KIT ,Mouse membrane protein expressed in epithelial-like lung adenocarcinoma ELISA KIT ,Mouse family with sequence similarity 57 member A ELISA KIT

Mouse FAM57A ELISA KIT Range 0.156-10ng/ml

Intended use

Mouse FAM57A ELISA KIT allows for the in vitro quantitative determination of FAM57A concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Test principle      

The microtiter plate provided in Mouse FAM57A ELISA KIT has been pre-coated with an FAM57A antibody specific to FAM57A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for FAM57A and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain FAM57A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of FAM57A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20