Mouse Regulator of G-protein signaling 9, Rgs9 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Mouse Rgs9 ELISA KIT ,Mouse PERRS ELISA KIT ,Mouse RGS9L ELISA KIT ,Mouse regulator of G-protein signalling 9 ELISA KIT ,Mouse regulator of G-protein signaling 9 ELISA KIT
Intended use
Mouse Rgs9 ELISA kit allows for the in vitro quantitative determination of Mouse Rgs9 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Mouse Rgs9 ELISA kit has been pre-coated with an antibody specific to Rgs9. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Rgs9 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rgs9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rgs9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 0.31-20ng/ml
Mouse Rgs9 ELISA kit Manual download
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