Mouse Retrotransposon- like protein 1, Rtl1 ELISA KIT

Product Name:Mouse Retrotransposon- like protein 1, Rtl1 ELISA KIT
Packing:96T                    Manual

Catalog No.:ELI-45322m

Gene Name:Mouse Rtl1

Detect Range: 31.25 pg/mL - 2000 pg/mL. The standard curve concentrations used for the ELISA’s were 2000 pg/mL, 1000 pg/mL, 500 pg/mL, 250 pg/mL, 125 pg/mL, 62.5 pg/mL, 31.25 pg/mL, 0 pg/mL.

Sensitivity:  The limit of detection of Mouse RTL1 defined as the analyte concentration resulting in an absorbance significantly higher than that of the dilution medium (mean plus 2 standard deviations) was determined to be 15.6 pg/mL (mean of 6 independent assays).

Target Protein Name:Mouse Rtl1 (Mouse Retrotransposon-like protein 1)

Target Protein Description:

The retrotransposon-derived mouse gene Rtl1 (retrotransposon-like-1), also known as Peg11 (paternally expressed-11) is a paternally expressed imprinted gene highly expressed at the late fetal stage in both the fetus and placenta. Rtl1 has an overlapping maternally expressed antisense transcript, Rtl1 antisense 4 (Rtl1as, also known as anti-Peg11), which contains several microRNAs (miRNAs) targeting the Rtl1 transcript through an RNA interference (RNAi) mechanism. Rtl1 is essential for maintenance of the fetal capillaries and that both its loss and its overproduction cause late fetal and/or neonatal lethality in mice. The observations contributed to the understanding of the formation and maintenance of the eutherian chorioallantoic placenta, and how the feto-maternal interface is maintained.

Alternative Name:Mouse Retrotransposon-like protein 1, Rtl1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Retrotransposon- like protein 1, Rtl1 ELISA KIT allows for the in vitro quantitative determination of Mouse Rtl1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Retrotransposon- like protein 1, Rtl1 ELISA KIT has been pre-coated with an Mouse Retrotransposon-like protein 1, Rtl1 antibody specific to Mouse Rtl1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rtl1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rtl1 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rtl1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!