Mouse Rho GTPase- activating protein 33, Arhgap33 ELISA KIT
Product Name:Mouse Rho GTPase- activating protein 33, Arhgap33 ELISA KIT
Packing:96T
Catalog No.:ELI-19304m
Gene Name:Mouse Arhgap33
Detect Range:0.15-10ng/ml
Sensitivity:0.094ng/ml
Target Protein Name:Mouse Arhgap33
Alternative Name:Mouse Rho GTPase-activating protein 33, Arhgap33
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse Rho GTPase- activating protein 33, Arhgap33 ELISA KIT allows for the in vitro quantitative determination of Mouse Arhgap33 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse Rho GTPase- activating protein 33, Arhgap33 ELISA KIT has been pre-coated with an Mouse Rho GTPase-activating protein 33, Arhgap33 antibody specific to Mouse Arhgap33 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Arhgap33 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Arhgap33 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Arhgap33 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!