Mouse RING finger protein 214, Rnf214 ELISA KIT
Product Name:Mouse RING finger protein 214, Rnf214 ELISA KIT
Packing:96T
Catalog No.:ELI-44966m
Gene Name:Mouse Rnf214
Detect Range:78.1-5000pg/ml
Sensitivity:46.9pg/ml
Target Protein Name:Mouse Rnf214
Alternative Name:Mouse RING finger protein 214, Rnf214
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Mouse RING finger protein 214, Rnf214 ELISA KIT allows for the in vitro quantitative determination of Mouse Rnf214 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Mouse RING finger protein 214, Rnf214 ELISA KIT has been pre-coated with an Mouse RING finger protein 214, Rnf214 antibody specific to Mouse Rnf214 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Rnf214 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Rnf214 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Rnf214 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!