Mouse S- methyl- 5'- thioadenosine phosphorylase, Mtap ELISA KIT

Product Name:Mouse S- methyl- 5'- thioadenosine phosphorylase, Mtap ELISA KIT
Packing:96T

Catalog No.:ELI-14201m

Gene Name:Mouse Mtap

Detect Range:78-5000pg/ml

Sensitivity:46.9pg/ml

Target Protein Name:Mouse Mtap

Alternative Name:Mouse S-methyl-5'-thioadenosine phosphorylase, Mtap
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse S- methyl- 5'- thioadenosine phosphorylase, Mtap ELISA KIT allows for the in vitro quantitative determination of Mouse Mtap concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse S- methyl- 5'- thioadenosine phosphorylase, Mtap ELISA KIT has been pre-coated with an Mouse S-methyl-5'-thioadenosine phosphorylase, Mtap antibody specific to Mouse Mtap .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Mtap and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Mtap , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Mtap in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!