Mouse Sarg ELISA KIT

Mouse Specifically androgen- regulated gene protein, SARG ELISA Kit

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

SARG, C1orf116,specifically androgen-regulated gene protein; specifically androgen-regulated protein; chromosome 1 open reading frame 116

 

Search name

Mouse SARG ELISA KIT ,Mouse C1orf116 ELISA KIT ,Mouse specifically androgen-regulated gene protein ELISA KIT ,Mouse specifically androgen-regulated protein ELISA KIT ,Mouse chromosome 1 open reading frame 116 ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Specifically androgen- regulated gene protein, SARG concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to SARG. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SARG and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SARG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SARG in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

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