Mouse Serine protease hepsin, Hpn ELISA KIT

Product Name:Mouse Serine protease hepsin, Hpn ELISA KIT
Packing:96T                                                                                              Manual

Catalog No.:ELI-05969m

Gene Name:Mouse Hpn

Detect Range:125-8000pg/ml

Sensitivity:62.5 pg/ml

Target Protein Name:Mouse Hpn

Alternative Name:Mouse Serine protease hepsin, Hpn
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Serine protease hepsin, Hpn ELISA KIT allows for the in vitro quantitative determination of Mouse Hpn concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Serine protease hepsin, Hpn ELISA KIT has been pre-coated with an Mouse Serine protease hepsin, Hpn antibody specific to Mouse Hpn .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Hpn and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Hpn , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Hpn in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!