Mouse Serine protease inhibitor Kazal- type 4, Spink4 ELISA KIT

Product Name:Mouse Serine protease inhibitor Kazal- type 4, Spink4 ELISA KIT
Packing:96T

Catalog No.:ELI-48796m

Gene Name:Mouse Spink4

Detect Range:78.1-5000pg/ml

Sensitivity:46.875pg/ml

Target Protein Name:Mouse Spink4

Alternative Name:Mouse Serine protease inhibitor Kazal-type 4, Spink4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Mouse Serine protease inhibitor Kazal- type 4, Spink4 ELISA KIT allows for the in vitro quantitative determination of Mouse Spink4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Mouse Serine protease inhibitor Kazal- type 4, Spink4 ELISA KIT has been pre-coated with an Mouse Serine protease inhibitor Kazal-type 4, Spink4 antibody specific to Mouse Spink4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Mouse Spink4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Mouse Spink4 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Mouse Spink4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!