Mouse Soluble Urokinase plasminogen activator surface receptor,SuPAR ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

sPLAUR,sMO,sCD87,soluble Urokinase plasminogen activator surface receptor,sU-PAR,suPAR, soluble Monocyte activation antigen Mo3,sURKR; soluble u-plasminogen activator receptor form 2; soluble urokinase plasmin

Search name

Mouse suPAR ELISA KIT ,Mouse sPLAUR ELISA KIT ,Mouse sMO ELISA KIT ,Mouse sCD87 ELISA KIT ,Mouse soluble Urokinase plasminogen activator surface receptor ELISA KIT ,Mouse sU-PAR ELISA KIT ,Mouse  soluble Monocyte activation antigen Mo3 ELISA KIT ,Mouse sURKR ELISA KIT ,Mouse soluble u-plasminogen activator receptor form 2 ELISA KIT ,Mouse soluble urokinase plasmin ELISA KIT

Intended use

Mouse suPAR ELISA KIT allows for the in vitro quantitative determination of mouse soluble Urokinase plasminogen activator surface receptor,SuPAR concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Mouse suPAR ELISA KIT has been pre-coated with an antibody specific to SuPAR. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for SuPAR and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SuPAR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SuPAR in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 0.15-10ng/ml

Mouse suPAR ELISA KIT PDF Manual download

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