Mouse TIRAP ELISA KIT

Mouse Toll/interleukin- 1 receptor domain- containing adapter protein, TIRAP ELISA KIT

96 Tests

Operating instruction

 

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

 

Synonyms

TIRAP,BACTS1; Mal; MyD88-2; wyatt; MyD88 adapter-like protein; Toll-like receptor adaptor protein; adapter protein wyatt; adaptor protein Wyatt; toll/interleukin-1 receptor domain-containing adapter protein; toll-interleukin 1 receptor (TIR) domain containing adaptor protein

 

Search name

Mouse TIRAP ELISA KIT ,Mouse BACTS1 ELISA KIT ,Mouse Mal ELISA KIT ,Mouse MyD88-2 ELISA KIT ,Mouse wyatt ELISA KIT ,Mouse MyD88 adapter-like protein ELISA KIT ,Mouse Toll-like receptor adaptor protein ELISA KIT ,Mouse adapter protein wyatt ELISA KIT ,Mouse adaptor protein Wyatt ELISA KIT ,Mouse toll/interleukin-1 receptor domain-containing adapter protein ELISA KIT ,Mouse toll-interleukin 1 receptor domain containing adaptor protein ELISA KIT ,Mouse TIR domain containing adaptor protein ELISA KIT

 

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Mouse Toll/interleukin- 1 receptor domain- containing adapter protein, TIRAP concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

 

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to TIRAP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TIRAP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TIRAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TIRAP in the samples is then determined by comparing the O.D. of the samples to the standard curve.