Mouse Transketolase, TKT ELISA Kit


  • Model: ELA-E0113m
  • 20 Units in Stock
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Mouse Transketolase, TKT ELISA Kit


96 Tests


Alternative name

Mouse TKT ELISA KIT ,Mouse TK ELISA KIT ,Mouse Transketolase ELISA KIT ,Mouse HEL-S-48 ELISA KIT ,Mouse HEL107 ELISA KIT ,Mouse TKT1 ELISA KIT ,Mouse epididymis luminal protein 107 ELISA KIT ,Mouse epididymis secretory protein Li 48 ELISA KIT


Intended use

Mouse TKT ELISA kit allows for the in vitro quantitative determination of Mouse TKT concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.


Test principle

The microtiter plate provided in Mouse TKT ELISA kit has been pre-coated with an antibody specific to TKT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TKT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TKT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TKT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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