Mouse Triosephosphate isomerase,TIM ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

TPI1,triosephosphate isomerase 1; triosephosphate isomerase; HEL-S-49; TIM; TPI; TPID; epididymis secretory protein Li 49; triose-phosphate isomerase

Search name

Mouse TPI1 ELISA KIT ,Mousetriosephosphate isomerase 1 ELISA KIT ,Mouse triosephosphate isomerase ELISA KIT ,Mouse HEL-S-49 ELISA KIT ,Mouse TIM ELISA KIT ,Mouse TPI ELISA KIT ,Mouse TPID ELISA KIT ,Mouse epididymis secretory protein Li 49 ELISA KIT ,Mouse triose-phosphate isomerase ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse triosephosphate isomerase,TIM concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to TIM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TIM and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TIM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TIM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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