Mouse Transient receptor potential cation channel subfamily V member 1, TRPV1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TRPV1,VR1; OTRPC1; capsaicin receptor; osm-9-like TRP channel 1; transient receptor potential cation channel subfamily V member 1; transient receptor potential vanilloid 1a; transient receptor potential vanilloid 1b; vanilloid receptor subtype 1; transient receptor potential cation channel subfamily V member 1, Vanilloid receptor 1,Capsaicin receptor
Search name
Mouse TRPV1 ELISA KIT ,Mouse VR1 ELISA KIT ,Mouse OTRPC1 ELISA KIT ,Mouse capsaicin receptor ELISA KIT ,Mouse osm-9-like TRP channel 1 ELISA KIT ,Mouse transient receptor potential cation channel subfamily V member 1 ELISA KIT ,Mouse transient receptor potential vanilloid 1a ELISA KIT ,Mouse transient receptor potential vanilloid 1b ELISA KIT ,Mouse vanilloid receptor subtype 1 ELISA KIT ,Mouse transient receptor potential cation channel subfamily V member 1 ELISA KIT ,Mouse Vanilloid receptor 1 ELISA KIT ,Mouse Capsaicin receptor ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of mouse transient receptor potential cation channel subfamily v member 1,TrpV1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to TrpV1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TrpV1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TrpV1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TrpV1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.