Mouse U4/U6.U5 tri- snRNP- associated protein 2, Usp39 ELISA KIT

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  • Model: ELI-13924m
  • 20 Units in Stock
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Mouse USP39 ELISA KIT

Packing  96Tests

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

Gene Name  USP39

Protein Name  U4/U6.U5 tri- snRNP- associated protein 2

Alternative Name

Mouse USP39 ELISA KIT ,Mouse 65K ELISA KIT ,Mouse CGI-21 ELISA KIT ,Mouse HSPC332 ELISA KIT ,Mouse SAD1 ELISA KIT ,Mouse SNRNP65 ELISA KIT ,Mouse U4/U6.U5 tri-snRNP-associated protein 2 ELISA KIT ,Mouse SAD1 homolog ELISA KIT ,Mouse SnRNP assembly defective 1 homolog ELISA KIT ,Mouse U4/U6.U5 tri-snRNP-associated 65 kDa protein ELISA KIT ,Mouse inactive ubiquitin-specific peptidase 39 ELISA KIT ,Mouse small nuclear ribonucleoprotein 65kDa (U4/U6.U5) ELISA KIT ,Mouse ubiquitin specific protease 39 ELISA KIT ,Mouse ubiquitin specific peptidase 39 ELISA KIT

Mouse USP39 ELISA KIT Range  78-5000pg/mL

Intended use

Mouse USP39 ELISA KIT allows for the in vitro quantitative determination of USP39 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

 

Reagent

Quantity

Assay plate

1

Standard

2

Sample Diluent

1 × 20mL

Assay Diluent A

1 × 10mL

Assay Diluent B

1 × 10mL

Detection Reagent A

1 × 120μL

Detection Reagent B

1 × 120μL

Wash Buffer(25 x concentrate)

1 × 30mL

Substrate

1 × 10mL

Stop Solution

1 × 10mL

Plate sealer

5

 

Test principle      

The microtiter plate provided in Mouse USP39 ELISA KIT has been pre-coated with an USP39 antibody specific to USP39. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for USP39 and then avidin conjugated to Horseradish Peroxidase (HRP) is added  to  each  microplate  well  and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain USP39, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of USP39 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

 

Sample collection and storage 

Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20

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